Modification of α-amylase fromBacillus licheniformisby the polyaldehyde derived from β-cyclodextrine and α-amylase thermostability

Amylase α-2A Autoantibodies

OBJECTIVE The pathogenesis of autoimmune pancreatitis (AIP) and fulminant type 1 diabetes remains unclear, although it is known that immune-mediated processes severely compromise the endocrine and exocrine functions in both diseases. RESEARCH DESIGN AND METHODS We have screened a lambdaTriplEx2 human pancreas cDNA library with serum from a patient with AIP and obtained positive clones. Sequen...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Production ofHalothermotolerant α-Amylase from aModerately Halophilic Bacterium, NesterenkoniaStrain F.

Production of extracellular amylase was demonstrated under conditions of high salinity in aerobically cultivated culture of a newly isolated moderately halophilic Gram-positive coccus, designated strain F in basal medium containing peptone from meat, yeast extract, NaCl (7% w/v) and starch. Biochemical and physiological characterization along with 16S rRNA sequence analysis placed F in the genu...

Inhibitory Effects of Swietenia Mahagoni Seeds Extract on Α-Glucosidase and Α-amylase

This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays.  Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C.  The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays.  A...

Molecular Engineering of the Geobacillus stearothermophilus α-Amylase and Cel5E from Chlostridium thermocellim; In Silico Approach

Background: Considering natural thermal stability, Geobacillus stearothermophilus amylase and Cel5E from Clostridium thermocellum are good candidates for industrial applications. To be compatible with the industrial applications, this enzyme should be stable in the high temperatures, so any improvement in their thermal stability is valuable.Objectives: Us...